Rbcl antibody

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August undefined, 2024

WebRibulose bisphosphate carboxylase large chain, RuBisCO large subunit, rbcl. Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana RBCL (AtCg00490). Antibodies were purified by affinity purification using immunogen. WebBackground: Patients undergoing kidney transplantation (KT) often receive red blood cell (RBC) transfusion during admission for KT which may increase the risk of allosensitization. The association between peri-transplant RBC transfusion and graft survival was evaluated using a nationwide cohort. Methods: This retrospective study analyzed 13,871 patients …

(CAC11076) rbcL Antibody - Biomatik - CiteAb

WebBcl-2 Antibody (C-2) is an IgG 1 κ mouse monoclonal Bcl-2 antibody (also designated BCL2 antibody or PPP1R50 antibody) that detects the Bcl-2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Bcl-2 Antibody (C-2) is available as both the non-conjugated anti-Bcl-2 antibody form, as well as multiple conjugated forms of anti-Bcl-2 … WebRBCL antibody (ABIN93539). Validated for CM, IF, Lbl. Tested in Arabidopsis thaliana, Baculogypsina sphaerulata. English +1 877 302 8632; Contact; Login Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 … dating chat forum

Plants Free Full-Text DNA Barcoding Study of Representative …

WebBcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). WebAntibodies for WB, IF, IHC, IP/ChIP of cucumber rbcL (rbcL, Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, Fragment, A5X4C8, NULL) WebAug 1, 2024 · The IP and WB assays using a pan-anti-Khib antibody verified that RbcL was indeed Khib-modified (Figure 4 A). The majority of Khib sites occur in the key function regions [46]. For example, the RbcL ε-amino groups, K175, K177, K201, and K252, play an important role in maintaining the stability of the -CO 2-Mg 2+ ternary complex [47]. bjs martin county fla

Anti-Rubisco (RbcL) antibody (Rabbit Polyclonal IgG)

WebJul 21, 2024 · To determine rbcL overexpression at the protein-level, we quantified RbcL protein from crude cell lysates by Western blot using rabbit anti-RbcL antibody (see Materials and Methods). In agreement with RuBisCO overexpression indicated by the RT-qPCR results, densitometric analyses revealed a mean ∼2 to 4-fold increase of RbcL … WebApr 11, 2024 · (RbcL) antibody also showed that the overexpression of OsRCA did not signiﬁcantly affect. the expression of Rubisco content (Figure 1 B). 2.3. Gas Exchange Parameters of Transgenic Lines. bjs matress brandWebSenior Research Scientist. University of Virginia. Oct 2024 - Mar 20242 years 6 months. Charlottesville, Virginia. Antibody mediated Immune Regulation (AMIR) • Understanding the mechanism of IgG ... bjs meat cutter pay

"WebAntibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome. For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, ( AS03 037) combined with Rubisco ready to use standard ( AS01 017) is recommended. " - Rbcl antibody

Rbcl antibody

RbcL Rubisco large subunit, form I (chicken) - Agrisera

WebImmunogen: ATCG00490 O03042 Synonyms: RbcL, Ribulose-1,5-bisphosphate carboxylase, oxygenase Background: Ribulose-1,5-bisphosphate carboxylase/oxygenase commonly known by the abbreviation RuBisCO, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants to energy … WebFeb 8, 2024 · Identifying this fact is a huge step forward, but the trouble is that proteins number in the tens of thousands and conventional small-molecule and antibody drugs can only access about 20% of the proteins we produce. The arrival of PROTACs. In 2001, an entirely new mechanism was proposed, protein-targeting chimeric molecules or PROTACs.

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WebJul 7, 2024 · (D and E) Comparison of the Rubisco amount in WT and Δraf1 by SDS-PAGE (D) and BN-PAGE (E), followed by immunoblot analysis using the anti-RbcL antibody. RbcL assemblies in Δraf1 exist predominantly in the form of L 8 S 8, with the same molecular weight as those in WT. No RbcL 2 or RbcL 8 assemblies were detectable at the bottom of … WebThe rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. The kit contains anti-RbcL antibody, recombinant RbcL protein and PEB buffer for quantitative protein extraction. Application Information. Western blot 1: 5,000-10,000 with standard ECL.

WebNov 2, 2024 · In addition, the solubility of RbcL was tested by fractionation of the total protein extracts using centrifugation at 12,000 rpm for 10 mins. Equal volumes of the supernatant and pellet resuspension that was resuspended in the same volume as the supernatant was loaded on SDS-PAGE, followed by immunoblot analysis using anti-RbcL … WebPolyclonal antibodies against rbcL and rbcS subunit were generated in female BALB/c mice and had no cross-reaction with each other. A total of 81 microg antigens were used and 0.3 ml anti-sera with titer of 1:5000 were yielded. The antibodies were also applicable to study rbcL and rbcS in tobacco plant Nicotiana benthamiana.

WebMar 9, 2024 · We employed primary antibodies for RubisCO protein, ubiquitin, and cellular proteins. Other primary antibodies were as follows: anti-RbcL (Agrisera), anti-His3 (Agrisera), ... WebToxin-speciﬁc antibodies were isolated from a full-length human IgG1 antibody library using an in vitro yeast selection system and associated methods.25-29 Toxin-binding mAbs were enriched by incubating biotin labeled Hla or leukocidin mono-mers with antibody expressing yeast cells at different concentra-

WebrbcL antibody, Ribulose bisphosphate carboxylase large chain antibody, RuBisCO large subunit antibody, EC 4.1.1.39 antibody. Protein Names Ribulose bisphosphate carboxylase large chain, RBL_SOYBN. UniProt Code History P27066, Q2PMV1. Gene Synonyms rbcL. View additional product information.

bjsm authorsWebApr 10, 2024 · The patient had no history of taking drugs that might cause PRCA, such as azathioprine, phenytoin, isoniazid or rifampicin. Parvovirus-B19 IgM antibodies and serological tests for hepatitis viruses (A, B, and C), human immunodeficiency virus, and Epstein-Barr virus (potential causes of PRCA) revealed negative results. bjs mattresses and box springWebRbcL / Anti-RbcL subunit of RuBisCO antibody: PHY0097S: FeSOD / Anti-Chloroplastic Fe-dependent superoxide dismutase antibody: PHY0104: RbcL / Anti-Rubisco large subunit, form I antibody: PHY0105S: ELIP2 / Anti-Early light inducible protein 2 antibody: PHY0127A: PGL35 / Anti-Plastoglobulin 35 antibody: bjsm bone healthWebWestern Blot analysis of RBCL using a RBCL Polyclonal antibody ( Product # PA5-98369 ). Positive WB detected in: Arabidopsis thaliana leaf tissue, Chlamydomonas tissue, Green alga tissue, Chlamydomonas reinhardtii tissue. All lanes: rbcL antibody at 1:1250. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. bjs meal replacement shakesWebJul 9, 2008 · Spherical bodies containing RbcL (RCBs; arrowheads) were found in the vacuolar compartment. B and C, Leaf sections were double immunolabeled with anti-GFP and anti-RbcL antibodies. After immunolabeling with anti-GFP antibodies followed by 15-nm gold-conjugated goat anti-rabbit IgG, silver enhancement of gold particles was performed. bjs meal prep containersWebThis antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. This antibody is not suitable for use in immunoprecipiation. This product can be sold containing ProClin if requested. dating chat discordWebThe RbcL protein standard can therefore be used in combination with global anti-RbcL antibodies to quantitate RbcL from a wide range of species.application informationrecommended dilutionStandard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a RbcL signal in … bjs mediterranean bowl