Phic31 integrase system

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August undefined, 2024

WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the … WebThe PhiC31 Integrase System. Frequently used for gene therapy development and related applications; Non-viral transgene delivery; Single-step process; Single-copy integration; …

phiC31 Integrase Vector System

WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … WebPhiC31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to ... christopher alman

An optimized transgenesis system for Drosophila using germ-line ... - PNAS

Web4. mar 2016 · The phiC31 integrase approach for integration was demonstrated here in iPSC, a potentially therapeutic cell type due to the ability of iPSC to be differentiated into muscle cells [6,7]. ...... WebThe PhiC31 Integrase System Popularly used for gene therapy development and related applications, one of the major advantages of SBI’s PhiC31 system is the tight control over copy number—the majority of clones that undergo integration events have single insertions (Supporting Data, Figure 1), reducing the risks of insertional mutagenesis effects. Web1. dec 2006 · The phiC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of … christopher alsobrook memphis

Integration specificity of phage phiC31 integrase in the human

Double knock-in pig models with elements of binary Tet-On and phiC31 …

Web14. sep 2012 · The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target … Web7. máj 2013 · It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific … christopher allport deathWeb17. mar 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme also … getting an interest only mortgage uk

"Web28. feb 2014 · PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in … " - Phic31 integrase system

Phic31 integrase system

Construction of transgenic Drosophila by using the site

WebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … WebPinpoint & PhiC31 Integrase Systems Minicircle Technology Episomal Expression Vectors (EEV) Custom mRNA Synthesis Production Cold Fusion Cloning T Cell Research Tools …

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WebWe previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. WebThe objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection. Materials and Methods: In this experimental study, the application of phiC31 integrase system was evaluated for

WebϕC31 integrase can integrate a donor plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. The ϕC31 … WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Use the form below and we’ll get back to you within 24-hours, or call us toll free at … Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … NTA-based Exosome Characterization - PhiC31 Integrase Expression Plasmid … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … Lentivirus Production - PhiC31 Integrase Expression Plasmid System Biosciences Lentiviral Packaging Kits - PhiC31 Integrase Expression Plasmid System Biosciences How it works. Ordering RNA Services is easy. Step 1: Contact the services team …

Web• Developed phiC31 integrase system in Hydra to optimize transgenesis via site-specific recombination. • Designed DNA constructs for microinjections. • Mentored undergraduate students and ... WebTo overcome these problems, it is necessary to develop a site-specific integration system. It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the silkworm, we first examined the efficiency of recombination between the ...

WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 …

Web18. máj 2024 · Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression. Jin Q1, Yang X1, Gou S1, Liu X1, Zhuang Z1, Liang Y1, Shi H1, Huang J2, Wu H1, Zhao Y1, Ouyang Z1, Zhang Q1, Liu Z1, Chen F1, Ge W1, Xie J1, Li N1, Lai C1, Zhao X1, Wang J1, Lian M1, Li L1, Quan L1, … getting an ird number for a trustThe mode integration/resolution and inversion (INT/RES and INV) depend on the orientation of recombinase target sites (RTS), among these pairs of attP and attB. Section C indicates, in a streamlined fashion, the way recombinase-mediated cassette exchange (RMCE) can be reached by synchronous double-reciprocal crossovers (rather than integration, followed by resolution). getting an ip pin from irsWeb4. mar 2016 · Use of phiC31 Integrase for Constructing Transgenic Organisms One application of phage integrases that has been popular is the use of phiC31 integrase to place transgenes into the genome to construct transgenic organisms. Both pseudo att sites and authentic att sites have been used in this regard. getting an insurance license in nj